Experiment (3) – Imagining Broad Types of Connectivity

The next step was to Consider how discrete sites might  be connected/’networkedtogether – i.e. ‘real time’, ‘offline’ or abstract connectivity?

Another way of seeing the world was possible, one infinitely more vital and interconnected than any I had previously imagined. In their worlds, information pulsed beneath the ground and floated on the breeze, interactions pulsed and shifted to the rhythm of the seasons, and knowledge and understanding grew, slowly but sturdily, over decades and centuries.
Bridle, J., 2023, Ways of Being

Assuming points of interest within the artwork site will somehow link within the work I began to consider the many ways such connectivity might be developed between the sites – on a continuum between the literal and the abstract- remembering that outcomes should be in some way beneficial (ie regenerative) rather than extractive.

Part of the artworks gulley area (Image Keith Armstrong, 24/4/24)

I was also aware of the importance of looking beyond the site (remembering it as a Mortonian ‘mesh’) – given the interconnections – for example the rich, cool dark vine forests along Samford Creek might one day be akin to the gulley area maybe? Could these be what some have called ‘mother sites’ (see the ‘mother tree’ concept from the wood wide web – highly-connected hub trees who share their excess carbon and nitrogen through the mycorrhizal network with the understory seedlings, which can increase seedling survival) –  places of deep time that the art work site is heading (back) towards – sites that imagine the future elsewhere – potentially where the exchange of materials happens ?

 Options

It struck me that connectivity might be be framed by the diverse ways that plants communicate/procreate/signal/interconnect with each other and the broader atmosphere throughout their lifecycles. For example could we mimic the ways plants send out signals to each other like grass seed pollen (impossible odds you’d think to hit a target??) – suggesting chance but rich interactions between nodes – or could nodes somehow come together to create something summative?

In a transitional aesthetic, art supports human and other forms of life, often exploited through extraction. Rather than fulfilling an extractive aesthetic, can ecosystems be reconstructed without overreach, but through regenerative acts?
Mary Mattingly (Link to source)

Mattingly’s words also offered up some possibilities :

My intuition at that stage was to engage a different/arguably more appropriate metaphor of connectvity for the work than the regular computational node and network models (eg the one that  wood wide web analogy riffs off) – a model/concept that for me maps less comfortable with the profoundly entangled universes of a site such as this. This might include:

  • Line of sight (but assumed visual signalling not relevant)
  • Electronic (signalling/osmosis through tubes/fungal hyphae/liquids/plumbing)
  • Gaseous (Chemical signals/VOCs/root absorption)
  • Wind (pollinated/semi random anther and stigma
  • see further notes at the bottom of this post

These questions led me back to some of the thinking of Karen Barad and her particular quantum brand of new materialism.

Intr-action of light and solar pump panel, SERF back paddock, April 2024 (Image Keith Armstrong)

Barad reminds us of a foundational paradox of the universe: that according to Quantum Physics, all matter paradoxically exhibits properties of both waves and particles.  Everything from light to compound molecules are according to  science observable both as particles—unique, finite, and (in most cases) material, and yet also as waves–in essence oscillations that carry energy as they propagate through media.  

A wave is therefore a form of energetic activity that in Barad’s words arises from/requires profoundly complex ‘intra-activity’ between particles – thereby forming shapes that self perpetuate and move through a medium – without being defined or entirely confined by that medium (think moire patterns or oceans).

Brad speaks to intra-activity as not two bodies acting upon one another but rather a process of becoming phenomena or becoming bodies. This suggests that the entire universe is a continual state of emergence – in which nothing is certain or fixed – but is always becoming itself through its intra-action with everything else. The material world is the constant intra-action of particles – of electrons touching everything else.

Active area edge of forest, 2023 (Image Courtesy of QUT REF team)

Intra-action is a Baradian term used to replace ‘interaction,’ which necessitates pre-established bodies that then participate in action with each other. Intra-action understands agency as not an inherent property of an individual or human to be exercised, but as a dynamism of forces (Barad, 2007, p. 141) in which all designated ‘things’ are constantly exchanging and diffracting, influencing and working inseparably. Intra-action also acknowledges the impossibility of an absolute separation or classically understood objectivity, in which an apparatus (a technology or medium used to measure a property) or a person using an apparatus are not considered to be part of the process that allows for specifically located ‘outcomes’ or measurement. Source

IN SUM
At that stage I resolved that the work should in someway engage the (geo and bio) spheres as they inflict each site – and that the connectivity between sites should be/or speak to in some way forms of wave motion rather than movement of discrete elements. To use another analogy – rather than the interconnectors being thought of as a pollen, instead focus on the waves of air caused by changing pressures that can be indicated by such pollen particles caught within them..

At that stage I was also continuing to ask:
What does beneficial mean within a respectful human plant engagement – does this suggest facets of ‘gardening’ maybe that create optimal conditions for flourishing – but what happens once then process stops??

How also might the overall work tune in and itself evolve over lifecycles – such as annual or perennial?

What might birth, growth and death means for the artwork – initiation, development, atrophy, decomposition, reintegration and new life ?

Black Spear Grass at SERF (Image Keith Armstrong)

The Quail Turn

Intelligence is not something to test – but rather to recognise ..

The Quail Turn: A Project Pivot

EXCITINGLY in late April a rare Red backed button quail (Turnix maculosus) had been sighted by the SERF bird group/Peter Storer. These birds are rarely seen and poorly studied – being agents of disguise within their preferred wet grassland environments – with a call that is a soft repeated ‘oom-oom-oom-oom’ and with the end of each note ascending in pitch. They are most often seen breaking wildly  for cover when disturbed and flying elsewhere in the grassland. They are listed as vulnerable (to extinction in NSW), although not in Qld. The itinerant ones are thought to arrive Oct/Nov and likely leave for the North late Feb/March – but this year’s wet hot season has likely kept them longer. (It is likely that the birds had in this case left end of April – pre the slashing season at SERF which is later than normal).

Rare Red-backed button quail (Turnix maculosus) sigthed in the artwork site (Image Peter Storer)

By chance I met Peter Storer in the paddock with his binoculars looking for the quail in late April using call sounds – as I was working late in the afternoon on setting up key sites. During that time at dusk, when they are noted to be active.  At that time I spotted a dead juvenile red backed button quail at the edge of the long grass – indicating breeding had happened (they build nests in shallow depressions in the grasses) .. suggesting that great care should be taken entering any of the artwork’s long grassed areas esp. during nesting season.

Deceased Red-backed button quail at the artwork site (Image Keith Armstrong)

Given that the site has been in essence maintained as a grassland by slashing for decades – and that this had attracted a rare find – this raised questions as to whether regenerating the previously burnt gulley/regeneration area with trees, or indeed premature slashing of other parts of the property, may drive them away/destroy their nests/cause them to not stop in the area in the future?

Clearly this finding presaged a project turning point of some kind that would require consultation across a number of groups and the science team. It seemed that the idea of ‘active’ and ‘passive’ areas may now need to change.

Flying Quail captured at SERF (Image Gavin O’Meera/ebird https://media.ebird.org/catalog?taxonCode=rebbut2&mediaType=photo

Other sightings of the red backed button on e-Bird

(Other birds know to be at the site (ref. Peter Storer) were teh brown quail (quite common, the Golden-headed Cisticola (Cisticola exilis), Tawny Grassbird (Cincloramphus timoriensis), Brown Quail (Coturnix ypsilophora) and Sacred Kingfisher (Todiramphus sanctus)).

 

Experiment (2) Initial Actions/Imaginings On-site (Pre-quail turn)

 

Panorama of the artwork sites, mid April 2024, (Image Keith Armstrong)

“I can’t think of a single scientific study in the last few decades that has demonstrated that plants or animals are dumber than we think. It’s always the opposite…We keep revealing the fact that all kinds of creatures have a capacity to learn, to have memory, and that we’re at the edge of this wonderful evolution in really understanding the sentience of other beings.”
Robin Wall Kimmerer quoted by James Bridle

To begin the process of imagining an artwork able to ‘cooperatively engage’ with the rich intelligence of the site, I resolved to undertake the observational and listening actions:

Obviously the idea that an artwork could ‘benefit’ the ecological evolution of the site is a relative fiction that must acknowledge the limits of what we can actually know, or indeed assume we might be able to know about worlds we study/work within .  In a parallel plane fractal geometry reminds us that there is always more to discover the more you pay attention. Whilst we might expect more order and clarity, in reality ever-closer examination will likely reveal more unexpected variation, nuance and detail. Hence The more accurately you might try to ‘measure’, appraise or describe things, (in so many ways) the more unmeasurable, unappraisable or indescribable they will likely become.

Late afternoon light on the artwork edges (Image Keith Armstrong)


STEP 1: Engaging ‘Spheres’

Classical science tells us that everything can  be  understood as being part of  “spheres”: the so called Geosphere and Biosphere that form the land, water, living things and air – and the infinite complexity of their interactions influence factors such  as soil salinitybiodiversity, and landscape formation/composition.

Whilst whilst such conceptual sub divisions are clearly an further divideable abstraction, their value to me lay in reminding me that much that we might easily sense/see/measure/ is limited by our human scale, faculties, ambition, technology and willingness or otherwise to transform the site towards our ends. At best these spheres are therefore interlocking, leaky, and  ambiguous – but ideally some facet of each would factor into the forthcoming work.

https://www.geographyrealm.com/what-are-the-earths-systems/

Geosphere

  • “lithosphere” (land) – includes the rocks and soils (which we were already engaging with in a particular way through soil bacteria)
  • “hydrosphere” (earth’s liquid water – visibly present on the surface in the still wetland/gulley region of the site, in teh silos and in the air )
  • “atmosphere” (the air surrounding (and penetrating) the other spheres)
  • (N/A) ” cryosphere” (frozen regions, including both ice and frozen soil);

BIOSPHERE

  • (living things, both visible and non visible)

Initial Actions in Regards to the Geo and Biospheres on site:

I resolved to:
Walk the perimeters of the artwork site and make initial localised observations. Choose and sit down at sites of interest in the ‘passive’ and ‘active’ areas of the artwork, and observe and listen. Observe local plants or plant communities  of special interest – which will be temporal in some cases, and sustained in others. (e.g. on 16/4/24 I spotted Scented top grass and Black spear – mid/late summer species doing well in the wet and warmth of this years April – so some sites might flourish at sometimes and be dormant other times of year). Make sketches, think small – begin to consider how to build up deep ‘observations’.  Be, and don’t be, methodical.  Identify a possible series of representative sites to place future artwork elements  – in both the grassland (active) and gulley (passive) areas. To record these activities place custom markers at points of interest.

Scented Top Grass / Capillipedium spicigerum in profusion at SERF 16/4/24 North of Gulley Site (Image Keith Armstrong)

This action was initially completed on Tue 16 + Thurs 18 April – using star pickets for marking key sites (locations of interest/monitoring camera sites). Future intention to use coloured site markers to in the grass to determine paths/minimise disturbance. (e.g. I sat at places of interest (usually where a new tree was pushing through the grass as a place to start), over time just quietly observing – as a result of this action I experienced maybe my first ever hay fever that night, noticed the profusion of insects most notably arachnids, ragged re-growth, and softness under as the Fimbrystis slowly dries out to late April maturity).

Black Spear Grass / Heteropogon contortus at SERF 16/4/24 North of gulley site  in small, isolated groups (Image Keith Armstrong)

As the late afternoon wore on my quiet, solo process was surprised by an unexpected person moving on the other side of the rise within SERF’s boundary – walking slowly through the grasses as if searching for something. I’d already heard from site manager Marcus Yates of  a rare Quail siting in that area – but this chance meeting  was pivotal – and threw into doubt how which ways the project might use the site going forward. (see The Quail Turn post)

 

Setup [6] Initial Scientific Monitoring

During April/May 2024, consistent with SERF’s other monitoring programs, and the need to track progress scientifically on this project, I decided to  initially establish 1-2 trail cameras on site – as phenocams (vegetation change cameras) –  and acoustic monitoring.

Acoustic Monitoring

I determined in consultation with Dr. David Tucker that initially 1 Solar powered BioAcoustic Recorder (Solar BAR) should be deployed near the bottom of the passive plot where it meets the active plot – to record continuous audio data for later analysis – given these devices’ detection radii is quite wide on open pasture (>100m): The choice of this hardware is consistent with the equipment used by the Australian Acoustic Observatory which Dr. David Tucker is a member of – ensuring he will be able to help identify the call sounds.

Acoustic Spectrogram of Bird Sound (Image courtesy of Frontier Labs)

SolarBAR recorder + Dr. David Tucker and Prof Paul Roe (Australian Acoustic Observatory)
Visual Monitoring
Phenocams are digital cameras (usually trail cams more typically used to capture nocturnal animal movements) set up to capture photo time-lapse images of foliage (ranging from one per 30 mins to one per day) at the same times. Scientists use these continuous visual records to observe things like vegetation development, including flowering, fruiting, and leaf lifecycles. I this way they are able to generate quantitative measures of plant phenology (timings of cyclical or seasonal biological events, that might include flowering, migrations, egg laying or hibernation).
4.0cg Sy electronic Technical co ltd Trail Cam with 4G – one of the various cameras I have to hand

Aerial Monitoring

This would be a mixture of input from the QUT REF team who fly 35mm quality cameras – and my own Mavic 2 Zooms. Initial photos were taken on 24/4/24 showing some of the area put aside for the artwork.

A part of the artwork site, April 2024 (Image Keith Armstrong)

 

(Philosophy 3): Plants and ‘Intelligence’ / Being With Plants

The ‘Intelligence’ of Plants
The founder of the ‘plant neurobiology movement’ Stephan Mancuso takes the view that our ” fetishisation of (mammalian) neurons, as well as our tendency to equate behaviour (and intelligence) with mobility, keeps us from appreciating what plants can do.” (Quote source).

Indeed, because they don’t move like animals, plants must by necessity develop an extensive, relational, and nuanced understanding of their local environments – arguably far beyond that of mammals like us. Plants therefore have to find everything they needs where they are located – and must have the capacity to defend themselves, while all the while remaining fixed in place. They must also cultivate ‘vectors’ – eg insects, animals or the wind to move their pollen to reproduce.

Growth in process at the ‘passive regeneration’ artwork site (Image Keith Armstrong)

This “sessile life style,” as plant biologists term it, calls for an extensive and nuanced understanding of one’s immediate environment, since the plant has to find everything it needs, and has to defend itself, while remaining fixed in place. A highly developed sensory apparatus is required to locate food and identify threats.

Plants of course do frequently get eaten and so they would not want to have any irreplaceable organs like a brain or legs. To cope with this, plants therefore developed a modular design which can in some cases allow them to lose  to 90% of their body and yet still survive.  This an extraordinary capability and which has now parallel in the animal kingdom. “ Plants therefore have a level of resilience. that we can barely imagine”.

In this introductory talk Neuro-Botanist Dr. Stefano Mancuso presents intriguing evidence about how plants behave in what might be termed as ‘intelligent ways’ from a human perspective – i.e. fighting predators, maximising food opportunities. (It’s worth considering where/if his analysis falls into the trap of categorising intelligence as ‘like us’.)

(Excerpt from above video re root growth tips  – Stefano Mancuso: The roots of plant intelligence)

In his article in the New Yorker  ‘The Intelligent Plant’ Michael Pollan suggests that plants have evolved between fifteen and twenty distinct senses, including analogues of our five: smell and taste (i.e. they sense and respond to chemicals in the air or on their bodies); sight (they react differently to various wavelengths of light as well as to shadow); touch (a vine or a root “knows” when it encounters a solid object); and, it has been recently discovered, sound.

In a recent experiment, Heidi Appel, a chemical ecologist at the University of Missouri, found that, when she played a recording of a caterpillar chomping a leaf for a plant that hadn’t been touched, the sound primed the plant’s genetic machinery to produce defence chemicals. Another experiment, done in Mancuso’s lab and not yet published, found that plant roots would seek out a buried pipe through which water was flowing even if the exterior of the pipe was dry, which suggested that plants somehow “hear” the sound of flowing water. (Source)

The Revolutionary Genius of Plants: A New Understanding of Plant Intelligence and Behavior

Hence, whilst these are all further reasons to celebrate plants,  the  focus of this research will lie in discovering, listening to, celebrating and (where appropriate) encouraging the flourishing of the multiple forms of  ‘intelligence’ living at the artwork site, and its environs/atmospheres. This may include

    • Behaviours of plants (notably the emergent and established trees, grasses, sedges at the site)
    • Actions of related insects (various)
    • Changes in soil and soil bacteria (Notably in our case Purpureocillium)
    • Changes in atmospheres at the site

Being with Plants

Whilst plants are always in flux (often going through extraordinarily different phases where sometimes as much as 90% of their mature bodies can be become absent) – their fixated/sessile nature offers us an amazing opportunity to return to be with them, time after time. Plants therefore offer us an opportunity to be fully present with them. This idea is summed up  poetically by Ryan Shea in this podcast from the Nature Institute – in which he also reminds us that “the trees can’t actually grab your face and turn you towards them, so you have to do some of the work yourself.”

The Nature Institute https://www.natureinstitute.org/podcast/in-dialogue-with-nature

Philosophy (2): Setting Limits/Direction

Setting Limits/Direction

Setting limits is vital in any arts project – and certainly one of this potential scope. @ April 2024, I have elected to make the following choices to frame subsequent initial work.

Fieldwork at the ‘active regeneration’ artwork site by A/Prof Caroline Hauxwell’s students, March 2024 (Image Keith Armstrong)
The project's starting focus will predominantly be on:

1: Observing/'coming to terms' with the  plant, fungal and invertebrate life and the supporting atmospheres that are profoundly governing and directing the evolution of the biological life at the site. {i.e. the on-site/in range non-human intelligences}.

2: Investigating the multiple, entangled 'intelligences' perceivable on site: consistent with the driving concept that 'intelligence' (a notoriously difficult to define term) is just one among many ways of being in worlds; and that it is profoundly entangled - given that everything in the more than-human (and of human) world is hitched to everything else. Hence, intelligence 'in the field' should not be categorised or reduced to something that is necessarily 'like us'. 

3: Developing commonality with First Nations 'right ways' of knowing and understanding intelligence within Country.

4: Using learnings from 1 - 3 to frame direct and shape the imagined symbiotic art forms.

FAI will therefore attempt to avoid repeating theories/practices  that have often (reductively) chosen to categorise ‘intelligence’ within human terms, as a implicit pillar of humanity’s longstanding taxonomic and anthropocentric project.

FAI also recognises and values the unique values of Indigenous biocultural knowledge and practices, and intends that the project’s key questions should remain constant with ‘cross-cultural’, ‘two-way’ or ‘right-way’ knowledge making/re-reviving . (See this related interview on ethnobotany and biocultural knowledge with Dr. Gerry Turpin from my prior project Carbon_Dating).

These stated foci build on my past projects (like the native grasses themed project Carbon_Dating) to recognise plants in so many ways define our biocultural environments – and therefore our capacity to live and share worlds with them.

What’s in it For Us? vs. Rights to Be/Become

The FAI team are effectively therefore acting as ‘native plant (and other species) guardians’ – playing our small part in guaranteeing their capacity to flourish.

Plants are super-critical to our survival because they provide us with oxygen, sustenance, clothing, medicine and more. However, the flourishing of any of these non-humans should not simply be dependent on their utilitarian use. Plants, like all other non-human life, have their own rights to be and become, and on their own terms. (This idea is endemic to the mission statement of the Australian Earth Laws Alliance ‘rights of nature’)

Recognising the Rights of Nature in law, means that we reject the notion that nature is human property and we legally recognise the rights of the natural world to exist, thrive and evolve. Recognising that the natural world is just as entitled to exist and evolve as we are, necessarily changes the way humans act.

‘Rights of Nature’ is grounded in the recognition that humanity is just one member of the wider earth community, and that we have evolved with, and are dependent upon, a healthy, interconnected web of life on Earth. Rights of Nature laws create guidance for actions that respect this relationship.

Unexpected colours of introduced grass at the artwork site, 2024 (Image Keith Armstrong)

This assertion doesn’t suggest that we shouldn’t harvest or eat plants or use them in other ways: However critically we should act respectfully towards them – and consider them  as much more than a back grounding to our human worlds (something  Wandersee and Schussler noted in 1999 that stems from our innate ‘plant blindness’). 

FAI’s aim therefore is that the site/artwork will evolve predominantly according to its own needs – and not ones that necessarily suit humankind. Its worth noting that science based management decisions, such as weed control or replanting, are also therefore understood and planned within that altruistic process.

Plant Blindness? (Image Keith Armstrong)

Philosophy (1): Key Questions/Orientation

The intention of this series of posts is to develop some of the philosophical threads that will bind the project.


Forest Art Intelligence (FAI) comprises

    1.  A regenerating forest; which is also regarded as the project’s meta-artwork
    2. a series of planned, temporal actions that our art and science team take to encourage that forest’s regeneration.
    3. a series of on-site hybrid creations (named ‘Art Intelligences’) invented as the project unfolds that evolve to occupy their own ecological ‘niches’ that can assist supporting that forest’s growth.
    4. a public engagement strategy/campaign to
      – illuminate the forest’s unique processes of intelligent natural regrowth
      – promote an more inclusive definition of intelligence that engages with  the non-human world.
Ultra narrow depth of Iron Bark (Image Keith Armstrong)

The FAI project therefore poses the following key questions:

  • How to develop a series of speculative, embedded forms, called ‘Art Intelligences’, capable of growing and evolving alongside a regenerating forest that can ALSO benefit that forest’s health.
  • How might such Art Intelligences slowly find, and then occupy, their own intelligent ‘niches’, within that forest’s ecology
  • How might such artworks bring attention to the extraordinary non-human intelligences that underpin natural systems AND harness them to inspire and direct this experimental sci-art process.
Lab Extraction: The Price (Image Keith Armstrong)

Experiment (2): Soil Biology @ the Artwork Site

As noted in this prior post, it has been particularly exciting to learn from A/Prof Caroline Hauxwell’s (Faculty of Science, School of Biology & Environmental Science.) extensive knowledge of the site and its soil biology.  (We had also engaged in fruitful discussions with here prior about the setup and the direction of the regeneration project).

Dr. Hauxwell has been using adjacent areas at SERF and part of the artwork/revegetation site for her research experiments into beneficial soil fungi – which have an end outcome to reduce pasture dieback that is caused by mealy bugs. (The bug in question is the paspalum mealybug, Heliococcus summervillei – and her work is of particular relevance to the pasture industry given that these invasive bugs devour Buffalo grass which is a key, commercial pasture grass, (and interestingly a notable environmental weed in conservation contexts). To do this work Caroline and her research team have become experts in isolating fungi from soils of forests and pastures.

Collecting soil samples along transects at SERF – across the artwork site (Image Keith Armstrong)

Immediately before, and following the burn of the SERF wet gulley/artwork site A/Prof Hauxwell had initiated a periodic soil sampling regime to learn how the fungal composition of the site might change according to the burn – something which also seemed very relevant to us given we needed proxy ways to determine the improvement of soil and plant health as the artwork process evolves.

So on 11/3/24 Dr. Eleanor Velasquez and I joined the soil sampling process at SERF – to understand and observe more about the scientific methods underway.

Soil samples from the artwork zone, collected and kept cool (Image Keith Armstrong)
Soil samples from the artwork zone (Image Keith Armstrong)
Artwork/site survey team, March 2024 (Image Keith Armstrong)
Site sampling notation (Image Keith Armstrong)

As per the prior study, samples would be taken in transects across the whole site (5 points per transect spanning the strip that we burned last year). Her students and postgraduate team had been analysing those soil samples for the presence of Purpureocillium (see prior soil biology investigation post).

Purpureocillium culture
Purpureocillium lilacinum culture. (https://www.adelaide.edu.au/mycology/fungal-descriptions-and-antifungal-susceptibility/hyphomycetes-conidial-moulds/purpureocillium)

Purpureocillium lilacinum is commonly isolated from soil, decaying vegetation, insects, nematodes and as a laboratory contaminant. It is also a causative agent of infection in human and other vertebrates (Luangsa-ard et al. 2011). 

Purpureocillium lilacinum (Subcultures from broth) (Image Keith Armstrong)

During our discussions we learnt that she would be continuing this work into 20924 – and she invited Eleanor and I to join her class which we have now done on a few occasions. The intentions of her study were to pursue three themes ..

  1. Soil Ecology: The isolation of facultative root endophytes, particularly P. lilacinum, to determine the effects of burning on abundance and diversity, and diversity within P. lilacinum.
  2. Media Development: Development of media for the production of P. lilacinum variants as an inoculum (that is – a liquid solution of the fungi that roots can grow through to make them resistant to Mealy bug) – which leads to the third area of interest
  3. The Good Bugs: The application of P. lilacinum variants as an inoculant against pasture mealybug.
Keith And Dr. Eleanor Velaqsquez in the soil biology lab, March 2024 (Image Keith Armstrong)

For me it has been a fascinating return to the analogue chemistry methods I remember only from Year 10 (!) – and has included both sampling soil to  the required protocol at SERF and then observing its analysis, culturing and the isolation of so called ‘morphotypes’ (in essence any of a group of different types of individuals of the same species in a population/individual fungi) for further growth on agar plate cultures. (An agar plate is a petri dish that contains a growth medium solidified with agar, and is used to culture microorganisms).

Student preparing soil solution containing bacteria onto an Agar plate (Image Keith Armstrong)
Soil fungi experiments with A/Prof Caroline Hauxwell, 2024 (Image Keith Armstrong)

Video of Agar Plate spreading

Inoculation medium/broth with dissolved fungi – ready for applying to grass plant roots (Image Keith Armstrong)
A mixture of different fungi to be described and isolated. (Image Keith Armstrong)
Notes on the composition (numbers of morphotypes and characteristics) of the fungi cultured over the past week – recorded by a student (Image Keith Armstrong)

The descriptions alone are evocative and the structure, form and ‘intelligence’ of this form suggest fruitful investigation ahead!

Preparing agar plates for the second stage of growth (Image Keith Armstrong)

The exciting idea that Caroline has raised – which further cements the fruitful connections emerging between the arts and sciences, is “to do a longitudinal study of the soil microbiome by adapting the sampling this year (across the burn site) to compare the dryer slope with the burned gully below.

Her (and our) interest would therefore be to see the changes in soil fungal diversity over time, particularly once trees are established.”  At this stage she is looking to do this highly technical, lab based work over several years – which will allow this project an extraordinary look into the health of the soil as the artwork develops. Exciting times ahead – so many thanks to A/Prof Hauxwell for your kindness, interest and engagement on this co-beneficial process 🙂

Purpureocillium lilacinum (Subcultures from broth) (Image Keith Armstrong)

 

 

Experiment (1): Visual Transects

Transect walks are a common method in conservation biology (Walpole and Sheldon 1999) and are used in participatory rural appraisal methods to uncover local information across landscapes (Chambers 1994). Transect walks involve walking in a straight line for a pre-defined distance, recording geographic coordinates every fifty meters or so, and writing down the kinds of environmental features around that point.(source)

Black and white image of bush with grass heads in the foreground.
Sunset @ SERF, Passive Regeneration Area (Image Keith Armstrong)

The first few weeks of any project involve processes of listening, observing and thinking through – and even more with a site like this that can only be interrogated through experience. Obviously open experimentation is valuable as part of this.

Sunset Visitor (Image Keith Armstrong)

Transect walking is something that I’d done with scientists before on other projects before – and was something that we’d done during the survey of plants during the winter before this project began (see list below this post) – walking over a few 100 metres – with posts set in the ground for surveying every  50 meters?

Cablecam setup, SERF passive regeneration area, 28/2/24 (Image Keith Armstrong)

It struck me that I could so something similar with a cable mounted camera – in order to record vegetation along the entire line rather than at set markers : Sure there would be camera blur – but maybe a time-lapse process taking stills would work better – these were my first experiments..

This method used time-lapse: NB need to debug the start stop nature of the unit – but the capture of sunset – And sharper images per frame are handy – esp. for cataloguing/identification purposes

Side views – offer capacity to experiment with other focal planes along continuous transect


Passive Plot, Species Map 1/7/23

The following species map was gathered by David Tucker and Gabrielle Lebbink on 17/7/23. NB this was a dry season assessment – and further species were evident after rains by Feb 2024.

Species No. Species Provenance Life Form (Perrenial/‌Annual-Graminoid/‌Forb/‌Shrub/‌Climber)
1 Cynodon dactylon Exotic PG
2 Digitaria spp.
(awaiting seed head to confirm and id  species
Exotic PG
3 Centella asiatica Native PF
4 Hypochaeris radiata Exotic PF
5 Imperata cylindrica Native PG
6 Fimbristylis spp. PG
7 Ageratum houstonianum Exotic PF
8 Sporobolus spp creber Native PG
9 Bidens pilosa Exotic AF
10 Polygala paniculata Exotic
11 Eragrostis brownii Native PG
12 Arundinella nepalensis Native PG
13 Alloteropsis semialata Native PG
14 Lobelia purpurascens Native PF
15 Sonchus oleraceus Exotic AF
16 Paspalum notatum Exotic PG
17 Eremochloa bimaculata Native PG
18 Wahlenbergia gracilis Native AF
19 Epaltes australis Native PF
20 Schenkia australis Native AF
21 Melinis repens Exotic PG
22 Velleia spathulata Native PF
23 Polygala spp Native
24 Phyllanthus virgatus Native AF
25 Cheilanthes sieberi Native AF
26 Big weed paspalum Exotic PG
27 Apiaceae spp

Maybe Ranunculus inundatus

Exotic PF
28 Cymbopogon refractus Native PG
29 Phyllanthus spp big
30 Lantana camara Exotic S
31 Dianella caerulea Native PG
32 Parsonsia straminea Native C
33 Passiflora suberosa Exotic C
34 Senna pendula Exotic S
35 Lomandra multiflora Native PG
36 Drosera spathulata Native AF
37 Aristida queenslandicum Native PG
38 Leucopogon juniperinus Native S
39 Gomphrena celesoides Exotic PH

 

 

Setup (5): Soil Biology Investigations (1)

Following discussions with the team – notably Gabrielle Lebbink and Eleanor Velasquez – we began to open up a new thread around the possibilities of soil inoculation – that is – finding ways to move material from eco active parts of the existing forest to the new sites.  This investigation is fuelled by/consistent with the need for the design of the artwork work to be of positive net benefit to the ecology of the emerging forest.

Gabrielle supplied some great information about ‘Re-wilding microbial/soil based communities’:

Contos P, Wood JL, Murphy NP, Gibb H. Rewilding with invertebrates and microbes to restore ecosystems: Present trends and future directions. Ecol Evol. 2021;11:7187–7200. https://doi.org/10.1002/ece3.7597. Downloaded y on [02/02/2024].

This article offered us some pointers of how we may be able to enhance soil biodiversity (potentially a part of an art/science process of trslocation of materials from the current forest to teh regeneration zone).  I noted of that paper  ..

Re-wilding of Microbe Communities (Notes, KMA 7/2/24)

Notes from: Contos P, Wood JL, Murphy NP, Gibb H. Rewilding with invertebrates and microbes to restore ecosystems: Present trends and future directions. Ecol Evol. 2021;11:7187–7200. https://doi.org/10.1002/ece3.7597. Downloaded from https://onlinelibrary.wiley.com/doi/10.1002/ece3.7597 by National Health and Medical Research Council, Wiley Online Library on [02/02/2024].

CONTEXT:
Invertebrates/microbes are key drivers of landscape scale ecosystem functions such as nutrient recycling and carbon sequestration, but many of these may still be functionally absent decades after a restoration process. The aim of any restoration project should actually be to reinstate ecosystem function and reestablishment of self-organising communities; and yet restoration ecologists rarely consider invertebrates and microbes which these make up the vast bulk of biodiversity and may drive key ecosystem recovery processes. Currently there is limited use of this approach in re-wilding projects – and often poor understanding of its importance.

Stepwise restoration  – i.e. a species at a time – is becoming less realistic in a dynamic and rapidly changing world. Typical approaches to restoration involve plant only and plant and animal only approaches – which operate under what the authors call the ‘field of dreams paradigm’ –  i.e. that if you build the habitat they will come! Such attempts often therefore ignore the ‘unseen majority’.

Normally microbial species translocations are done for the benefit of other species (i.e. to encourage back butterflies or for the benefit of a particular plant health)) rather than for themselves per se (e.g. introduced AMF fungi) – without considering whole communities.  Furthermore, in most cases microbes are rarely monitored afterwards anyway. Hence some environments may remain functionally unrecovered 10 or 20 years later. NB The process of microbial re-wilding is more common in soil inoculation studies (soil transplants).

Diagram from paper showing how we might move material across the ‘Inhospitable Matrix’ that bounds the artwork site: “Leaf litter samples taken from remnant patches and moved into revegetation patches will carry a multitude of invertebrate and microbe species and individuals. Inset: detritivorous mites and springtails taken from a leaf litter sample”.

Whole of community microbial (and invertebrate?) rewilding is often practical due to ease of manipulation – e.g. in forest litter invertebrates and microbes (e.g. detritovores) are critical components – (see p7193). Whole of community rewilding typically involves transporting small subsets of whole habitats – normally from a nearby remnant site to a revegetation plot that maybe separated by an ‘inhospitable matrix’ – e.g. a pasture – and may include elements such as soil, dead wood and pond mud.

“Collecting soil from the whole rhizosphere region of large established trees is impractical. Collecting rhizosphere communities by sampling 1 m out from the base of trees using a soil corer is a viable methodologic approach and is a more targeted way of rewilding microbial communities than current soil inoculation studies. Previous research has demonstrated that rhizosphere signatures can be detected using this approach for microbe communities from rainforest plant species despite the complex overlapping root networks (Wood et al., 2020)”

Its critical to avoid failures which will result in overall net loss of biota in an already stressed environment). The authors therefore suggest considering:

  • suitability of renewed habitat to accommodate the microbes: The revegetation site should be ameliorated and receptive (physical and chemical limitations of soil) – and the source site very carefully scanned for invasives and pathogens.
  • dispersal limitations of some invertebrates and microbes
  • Distance of new site from existing vegetation ideally should not be far
  • May work better in cool wet conditions when microbes and invertebrates are active and closer to surface of litter level (n.b. authors outside Australia)

Broadly speaking a project should

  • Set restoration goals
  • Evaluate the restoration trajectory
  • Choose what to re-wild
  • Conduct post re-wilding monitoring
  • Evaluate success/failures.
“Litter communities contain a breadth of species, including trilobite cockroaches, Laxta granicollis (center), and armadillid isopods (top right). These taxa are often overlooked during rewilding projects, despite their immense contribution to biodiversity and their influence on ecosystem functions such as decomposition. Photo credit: L Menz”

See also this great podcast:  https://www.futureecologies.net/listen/life-in-the-soil

These interesting learnings   dovetailed with the research one of the other science academics  (A/Prof Caroline Hauxwell) who is investigating diversity of fungal communities in soil, including Purpureocillium, which is a interesting fungus associated with pasture health and resilience to pests and diseases. She is interested in the impacts of burning on beneficial fungal symbionts (and thus the artwork site is an ideal choice) – something that ties in with her work on management of the pasture mealybug Heliococcus summervillei, which causes ‘pasture dieback’ (research that is co-funded with Meat and Livestock Australia). A/Prof Hauxwell is one of Australia’s foremost experts in this area and her research is extensive and widely recognised.

Dr. Hauxwell had already independently set up an experiment to monitor the artwork areas pre and post burn – so we were all ears to her process! Generously she invited Eleanor and I to join her class and get involved in some of her soil science – we jumped at this opportunity as an interesting creative opening.

 

To get more of a handle on this work – here are some excerpts from an email that A/Prof Caroline Hauxwell subsequently wrote – as part of a group email  in Feb 2024.

We welcome, Keith Armstrong, who initiated the burn at SERF and who is very interested in this work. Welcome, Keith.

“For most of you the work really starts in week 3 with the field trip to collect soil samples at SERF.

The project work this year is a Deep Dive on Soil Endophytes. We’ll be focusing particularly on Purpureocillium lilacinum. The project builds on sampling that we did before and shortly after a burn site at SERF for capstone last year.

The student data identified changes in Purpureocillium abundance and distribution in the burned area.

Fungal culture (Image c/o Sean Martin, created with particiapnts at the 2021 Woodford Bio Lab)

We also know from our research that P. lilacinum isolates have significant morphological diversity, but ITS sequencing has shown only 1 sequence/variant. We hope to look a bit more closely at P. lilacinum diversity in this project. All groups will also have access to 3 PCR primers for Sanger sequencing: one to characterise fungi to genus (ITS) and 2 others to take a deep dive on the identity of P. lilacinum morphotypes. For reference see Naimul’s work on Metarhizium. (https://eprints.qut.edu.au/view/person/Islam,_Shah.html) and https://doi.org/10.1016/j.funeco.2022.101179.

Soil Ecologists: The isolation of facultative root endophytes, particularly P. lilacinum, the effects of burning on abundance and diversity, and diversity within P. lilacinum.

Media Types: Development of media for the production of P. lilacinum variants as an inoculum

Fungal culture (Image c/o Sean Martin, created with participants at the 2021 Woodford Bio Lab)

All students (plus artists and the science team) will take part in soil sampling .. at SERF. This will take soil samples at 5 points along a transect across a strip that was burned last year See picture attached). 2 sample points will be outside the burn, and 3 inside it. All  will plate out their soil samples onto selective media.

For further background of this process see a report of the 2021 lab run by A/Prof Caroline Hauxwell at Woodford

 

Recipient of ANAT Synapse Residency 2024